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Aim. The aim of this study was to investigate the effect of applying checklist on intra-hospital transport of intensive care patients. Background. Intra-hospital transport of intensive care patients who are at risk of cardiovascular and respiratory instability can lead to physical harm and death. Therefore, prevention through increasing awareness staff about the dangers is a necessity. Method. A quasi-experimental before-after design was used to conduct the study. At first, in a hospital affiliated to Tehran University of Medical Sciences, the quality of 50 cases of intra-hospital transport observed using a checklist during day shifts. Then, intra-hospital transport checklist was given to critical care nurses to be used in cases of patient transport. One month later, the quality of 50 cases of intra-hospital transport observed using a checklist during day shifts. The data were analyzed by SPSS version 21. Findings. The results showed a statistically significant difference between the mean score of the quality of intra-hospital transport, before and after intervention (49.26±9.87 against 54.95±10.27) (P=0.006). Conclusion. Applying checklist improves intra-hospital transport of intensive care patients. It is recommended to use this checklist for intra-hospital transport of intensive care patients to increase patient safety and reduce the complications of in-hospital transport .